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cd14 + selection macs purification  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd14 + selection macs purification
    Cd14 + Selection Macs Purification, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+++selection+macs+purification/cd14/pmc11064100-424-10-15
    Average 90 stars, based on 1 article reviews
    cd14 + selection macs purification - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Sepsis expands a CD39 + plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity.
    Article Snippet: .. Primary human CD14+ monocytes were purified by positive selection from PBMCs using CD14 MACS beads Multisort (Miltenyi Biotec) according to the manufacturer’s recommendations. ..

    Article Title: Bindarit Reduces Bone Loss in Ovariectomized Mice by Inhibiting CCL2 and CCL7 Expression via the NF‐κB Signaling Pathway
    Article Snippet: .. BMMs were collected from the Third Affiliated Hospital of Southern Medical University and aseptically purified immediately via centrifugation using monocyte isolation solution (Ficoll‐Paque PLUS, GE Healthcare, Chicago, IL, USA) and immunomagnetic selection (CD14 MicroBeads human, MACS, Miltenyi BiotecTM, Bergisch Gladbach , Germany) following the manufacturer's instructions. ..

    Article Title: Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing
    Article Snippet: We applied Ficoll-Paque Plus and diluted with PBS by density gradient centrifugation (400 g, 20 min, without break). .. Then, the monocytes were purified by positive selection with human CD14 + magnetic MicroBeads by manual MACS cell separation system (Miltenyi Biotec). ..

    Article Title: Intracellular pyruvate levels positively correlate with cytokine production capacity in tolerant monocytes from patients with pneumonia.
    Article Snippet: Isolation of PBMCs was performed by density-gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare, Chicago, IL). .. CD14+ monocytes were subsequently purified using MACS CD14 microbeads for positive selection, according to the manufacturer's instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Development of an In Vitro Assay to Assess Pharmacological Compounds and Reversion of Tumor-Derived Immunosuppression of Dendritic Cells.
    Article Snippet: Background: Cancer immunotherapies have achieved much success and have become the forefront treatment of cancers previously associated with poor prognosis.. However, a major challenge in cancer immunotherapies remains the heterogeneity of the immunoregulatory capacities of cancers, and not all patients of a given cancer responds to current therapeutic strategies.. To address this issue and to facilitate the development of new pharmacological compounds, we here describe an in vitro model of dendritic cell suppression by cancer cells.

    Article Title: Bone marrow-derived macrophages converted into cancer-associated fibroblast-like cells promote pancreatic cancer progression.
    Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) is characterized by a desmoplastic reaction caused by cancerassociated fibroblasts (CAFs), which provokes treatment resistance.. CAFs are newly proposed to be heterogeneous populations with different functions within the PDAC microenvironment.. The most direct sources of CAFs are resident tissue fibroblasts and mesenchymal stem cells, however, the origins and functions of CAF subtypes remain unclear.

    Selection:

    Article Title: Sepsis expands a CD39 + plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity.
    Article Snippet: .. Primary human CD14+ monocytes were purified by positive selection from PBMCs using CD14 MACS beads Multisort (Miltenyi Biotec) according to the manufacturer’s recommendations. ..

    Article Title: Bindarit Reduces Bone Loss in Ovariectomized Mice by Inhibiting CCL2 and CCL7 Expression via the NF‐κB Signaling Pathway
    Article Snippet: .. BMMs were collected from the Third Affiliated Hospital of Southern Medical University and aseptically purified immediately via centrifugation using monocyte isolation solution (Ficoll‐Paque PLUS, GE Healthcare, Chicago, IL, USA) and immunomagnetic selection (CD14 MicroBeads human, MACS, Miltenyi BiotecTM, Bergisch Gladbach , Germany) following the manufacturer's instructions. ..

    Article Title: Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing
    Article Snippet: We applied Ficoll-Paque Plus and diluted with PBS by density gradient centrifugation (400 g, 20 min, without break). .. Then, the monocytes were purified by positive selection with human CD14 + magnetic MicroBeads by manual MACS cell separation system (Miltenyi Biotec). ..

    Article Title: Intracellular pyruvate levels positively correlate with cytokine production capacity in tolerant monocytes from patients with pneumonia.
    Article Snippet: Isolation of PBMCs was performed by density-gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare, Chicago, IL). .. CD14+ monocytes were subsequently purified using MACS CD14 microbeads for positive selection, according to the manufacturer's instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Development of an In Vitro Assay to Assess Pharmacological Compounds and Reversion of Tumor-Derived Immunosuppression of Dendritic Cells.
    Article Snippet: Background: Cancer immunotherapies have achieved much success and have become the forefront treatment of cancers previously associated with poor prognosis.. However, a major challenge in cancer immunotherapies remains the heterogeneity of the immunoregulatory capacities of cancers, and not all patients of a given cancer responds to current therapeutic strategies.. To address this issue and to facilitate the development of new pharmacological compounds, we here describe an in vitro model of dendritic cell suppression by cancer cells.

    Article Title: Bone marrow-derived macrophages converted into cancer-associated fibroblast-like cells promote pancreatic cancer progression.
    Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) is characterized by a desmoplastic reaction caused by cancerassociated fibroblasts (CAFs), which provokes treatment resistance.. CAFs are newly proposed to be heterogeneous populations with different functions within the PDAC microenvironment.. The most direct sources of CAFs are resident tissue fibroblasts and mesenchymal stem cells, however, the origins and functions of CAF subtypes remain unclear.

    Magnetic Cell Separation:

    Article Title: Sepsis expands a CD39 + plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity.
    Article Snippet: .. Primary human CD14+ monocytes were purified by positive selection from PBMCs using CD14 MACS beads Multisort (Miltenyi Biotec) according to the manufacturer’s recommendations. ..

    Article Title: Bindarit Reduces Bone Loss in Ovariectomized Mice by Inhibiting CCL2 and CCL7 Expression via the NF‐κB Signaling Pathway
    Article Snippet: .. BMMs were collected from the Third Affiliated Hospital of Southern Medical University and aseptically purified immediately via centrifugation using monocyte isolation solution (Ficoll‐Paque PLUS, GE Healthcare, Chicago, IL, USA) and immunomagnetic selection (CD14 MicroBeads human, MACS, Miltenyi BiotecTM, Bergisch Gladbach , Germany) following the manufacturer's instructions. ..

    Article Title: Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing
    Article Snippet: We applied Ficoll-Paque Plus and diluted with PBS by density gradient centrifugation (400 g, 20 min, without break). .. Then, the monocytes were purified by positive selection with human CD14 + magnetic MicroBeads by manual MACS cell separation system (Miltenyi Biotec). ..

    Article Title: Intracellular pyruvate levels positively correlate with cytokine production capacity in tolerant monocytes from patients with pneumonia.
    Article Snippet: Isolation of PBMCs was performed by density-gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare, Chicago, IL). .. CD14+ monocytes were subsequently purified using MACS CD14 microbeads for positive selection, according to the manufacturer's instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Development of an In Vitro Assay to Assess Pharmacological Compounds and Reversion of Tumor-Derived Immunosuppression of Dendritic Cells.
    Article Snippet: Background: Cancer immunotherapies have achieved much success and have become the forefront treatment of cancers previously associated with poor prognosis.. However, a major challenge in cancer immunotherapies remains the heterogeneity of the immunoregulatory capacities of cancers, and not all patients of a given cancer responds to current therapeutic strategies.. To address this issue and to facilitate the development of new pharmacological compounds, we here describe an in vitro model of dendritic cell suppression by cancer cells.

    Article Title: Bone marrow-derived macrophages converted into cancer-associated fibroblast-like cells promote pancreatic cancer progression.
    Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) is characterized by a desmoplastic reaction caused by cancerassociated fibroblasts (CAFs), which provokes treatment resistance.. CAFs are newly proposed to be heterogeneous populations with different functions within the PDAC microenvironment.. The most direct sources of CAFs are resident tissue fibroblasts and mesenchymal stem cells, however, the origins and functions of CAF subtypes remain unclear.

    Centrifugation:

    Article Title: Bindarit Reduces Bone Loss in Ovariectomized Mice by Inhibiting CCL2 and CCL7 Expression via the NF‐κB Signaling Pathway
    Article Snippet: .. BMMs were collected from the Third Affiliated Hospital of Southern Medical University and aseptically purified immediately via centrifugation using monocyte isolation solution (Ficoll‐Paque PLUS, GE Healthcare, Chicago, IL, USA) and immunomagnetic selection (CD14 MicroBeads human, MACS, Miltenyi BiotecTM, Bergisch Gladbach , Germany) following the manufacturer's instructions. ..

    Isolation:

    Article Title: Bindarit Reduces Bone Loss in Ovariectomized Mice by Inhibiting CCL2 and CCL7 Expression via the NF‐κB Signaling Pathway
    Article Snippet: .. BMMs were collected from the Third Affiliated Hospital of Southern Medical University and aseptically purified immediately via centrifugation using monocyte isolation solution (Ficoll‐Paque PLUS, GE Healthcare, Chicago, IL, USA) and immunomagnetic selection (CD14 MicroBeads human, MACS, Miltenyi BiotecTM, Bergisch Gladbach , Germany) following the manufacturer's instructions. ..

    other:

    Article Title: Chemokine CXCL4 interactions with extracellular matrix proteoglycans mediate wide-spread non-receptor mediated immune cell recruitment
    Article Snippet: Monocytes were then purified from freshly isolated PBMCs using a CD14 + selection MACS purification (Miltenyi Biotec) followed by final re-suspension in chemotaxis buffer (Dulbecco’s modified Eagles medium (DMEM) (Sigma) + 10% (v/v) foetal bovine serum (FBS) (Sigma)).

    Article Title: Chemokine CXCL4 interactions with extracellular matrix proteoglycans mediate wide-spread immune cell recruitment independent of chemokine receptors
    Article Snippet: Monocytes were then purified from freshly isolated PBMCs using a CD14 + selection MACS purification (Miltenyi Biotec) followed by final re-suspension in chemotaxis buffer (DMEM (Sigma) + 10% (v/v) FBS (Sigma)).



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    Miltenyi Biotec cd14 + selection macs purification
    Cd14 + Selection Macs Purification, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+++selection+macs+purification/cd14/pmc11064100-424-10-15
    Average 90 stars, based on 1 article reviews
    cd14 + selection macs purification - by Bioz Stars, 2026-10
    90/100 stars
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